human premixed multi-analyte kit Search Results


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Human Premixed Multi Analyte Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PBL Assay verikine hs interferon alpha all subtype elisa kit
Fig. 1 INI-4001 is a synthetic TLR7/8 agonist. a Chemical structure of INI-4001. b, c SEAP activation in HEK293 cells expressing human TLR7 (b) or human TLR8 (c) by indicated doses of INI-4001. Data were reported as fold change in SEAP production over vehicle control and shown as mean ± SEM. d, e Human PBMCs from five different donors were stimulated with indicated doses of INI-4001 for 24 h. Supernatants were then analyzed for IFNα (d) and TNFα (e) production by <t>ELISA.</t>
Verikine Hs Interferon Alpha All Subtype Elisa Kit, supplied by PBL Assay, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human premixed multi analyte kits
Fig. 1 INI-4001 is a synthetic TLR7/8 agonist. a Chemical structure of INI-4001. b, c SEAP activation in HEK293 cells expressing human TLR7 (b) or human TLR8 (c) by indicated doses of INI-4001. Data were reported as fold change in SEAP production over vehicle control and shown as mean ± SEM. d, e Human PBMCs from five different donors were stimulated with indicated doses of INI-4001 for 24 h. Supernatants were then analyzed for IFNα (d) and TNFα (e) production by <t>ELISA.</t>
Human Premixed Multi Analyte Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human il
Fig. 1 INI-4001 is a synthetic TLR7/8 agonist. a Chemical structure of INI-4001. b, c SEAP activation in HEK293 cells expressing human TLR7 (b) or human TLR8 (c) by indicated doses of INI-4001. Data were reported as fold change in SEAP production over vehicle control and shown as mean ± SEM. d, e Human PBMCs from five different donors were stimulated with indicated doses of INI-4001 for 24 h. Supernatants were then analyzed for IFNα (d) and TNFα (e) production by <t>ELISA.</t>
Human Il, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems custom human premixed multi analyte kit
Fig. 1 INI-4001 is a synthetic TLR7/8 agonist. a Chemical structure of INI-4001. b, c SEAP activation in HEK293 cells expressing human TLR7 (b) or human TLR8 (c) by indicated doses of INI-4001. Data were reported as fold change in SEAP production over vehicle control and shown as mean ± SEM. d, e Human PBMCs from five different donors were stimulated with indicated doses of INI-4001 for 24 h. Supernatants were then analyzed for IFNα (d) and TNFα (e) production by <t>ELISA.</t>
Custom Human Premixed Multi Analyte Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1 INI-4001 is a synthetic TLR7/8 agonist. a Chemical structure of INI-4001. b, c SEAP activation in HEK293 cells expressing human TLR7 (b) or human TLR8 (c) by indicated doses of INI-4001. Data were reported as fold change in SEAP production over vehicle control and shown as mean ± SEM. d, e Human PBMCs from five different donors were stimulated with indicated doses of INI-4001 for 24 h. Supernatants were then analyzed for IFNα (d) and TNFα (e) production by ELISA.

Journal: NPJ vaccines

Article Title: A lipidated TLR7/8 adjuvant enhances the efficacy of a vaccine against fentanyl in mice.

doi: 10.1038/s41541-023-00694-y

Figure Lengend Snippet: Fig. 1 INI-4001 is a synthetic TLR7/8 agonist. a Chemical structure of INI-4001. b, c SEAP activation in HEK293 cells expressing human TLR7 (b) or human TLR8 (c) by indicated doses of INI-4001. Data were reported as fold change in SEAP production over vehicle control and shown as mean ± SEM. d, e Human PBMCs from five different donors were stimulated with indicated doses of INI-4001 for 24 h. Supernatants were then analyzed for IFNα (d) and TNFα (e) production by ELISA.

Article Snippet: IFNα was analyzed via VeriKine-HS Interferon Alpha All Subtype ELISA Kit (PBL Assay Science) according to the manufacturer’s instructions.

Techniques: Activation Assay, Expressing, Control, Enzyme-linked Immunosorbent Assay

Fig. 3 Adjuvanting F1-CRM with INI-4001 plus alum preferentially increases anti-F1 IgG2a antibody concentration. Mice were vaccinated twice, IM, with 5 µg F1-CRM plus 7.68, 9, or 22.5 µg alum, 0.1, 1, or 10 µg INI-2002, 0.1, 1, or 10 µg INI-4001, the combination of INI-2002 + alum, or the combination of INI-4001 + alum as indicated. Fourteen days after the second vaccination, blood was collected, and anti-F1 IgG (a, b), IgG1 (c, d), and IgG2a (e, f) antibody concentrations were measured by ELISA. Antibody responses to vaccines adjuvanted with INI-2002 are shown on the left (a, c, e). Antibody responses to vaccines adjuvanted with INI-4001 are shown on the right (b, d, f). Statistical analysis was conducted by one-way ANOVA with Fisher’s LSD for multiple comparisons (GraphPad Prism). *p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001; color of asterisks indicates comparison group.

Journal: NPJ vaccines

Article Title: A lipidated TLR7/8 adjuvant enhances the efficacy of a vaccine against fentanyl in mice.

doi: 10.1038/s41541-023-00694-y

Figure Lengend Snippet: Fig. 3 Adjuvanting F1-CRM with INI-4001 plus alum preferentially increases anti-F1 IgG2a antibody concentration. Mice were vaccinated twice, IM, with 5 µg F1-CRM plus 7.68, 9, or 22.5 µg alum, 0.1, 1, or 10 µg INI-2002, 0.1, 1, or 10 µg INI-4001, the combination of INI-2002 + alum, or the combination of INI-4001 + alum as indicated. Fourteen days after the second vaccination, blood was collected, and anti-F1 IgG (a, b), IgG1 (c, d), and IgG2a (e, f) antibody concentrations were measured by ELISA. Antibody responses to vaccines adjuvanted with INI-2002 are shown on the left (a, c, e). Antibody responses to vaccines adjuvanted with INI-4001 are shown on the right (b, d, f). Statistical analysis was conducted by one-way ANOVA with Fisher’s LSD for multiple comparisons (GraphPad Prism). *p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001; color of asterisks indicates comparison group.

Article Snippet: IFNα was analyzed via VeriKine-HS Interferon Alpha All Subtype ELISA Kit (PBL Assay Science) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Vaccines, Comparison

Fig. 4 The addition of INI-2002 to INI-4001 plus alum does not further increase F1-specific antibody concentrations. Mice were vaccinated twice, IM, with 5 µg F1-CRM plus 9, 22.5, or 24 µg alum, 1 µg INI-2002, 10 µg INI-4001, the combination of INI-2002 + INI- 4001, INI-2002 + alum, INI-4001 + alum, or INI-2002 + INI- 4001 + alum as indicated. Fourteen days after the second vaccina- tion, blood was collected and anti-F1 IgG (a), IgG1 (b), and IgG2a (c) antibody concentrations were measured by ELISA. Statistical analysis was conducted by one-way ANOVA with Fisher’s LSD for multiple comparisons (GraphPad Prism). *p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001; color of asterisks indicates comparison group.

Journal: NPJ vaccines

Article Title: A lipidated TLR7/8 adjuvant enhances the efficacy of a vaccine against fentanyl in mice.

doi: 10.1038/s41541-023-00694-y

Figure Lengend Snippet: Fig. 4 The addition of INI-2002 to INI-4001 plus alum does not further increase F1-specific antibody concentrations. Mice were vaccinated twice, IM, with 5 µg F1-CRM plus 9, 22.5, or 24 µg alum, 1 µg INI-2002, 10 µg INI-4001, the combination of INI-2002 + INI- 4001, INI-2002 + alum, INI-4001 + alum, or INI-2002 + INI- 4001 + alum as indicated. Fourteen days after the second vaccina- tion, blood was collected and anti-F1 IgG (a), IgG1 (b), and IgG2a (c) antibody concentrations were measured by ELISA. Statistical analysis was conducted by one-way ANOVA with Fisher’s LSD for multiple comparisons (GraphPad Prism). *p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001; color of asterisks indicates comparison group.

Article Snippet: IFNα was analyzed via VeriKine-HS Interferon Alpha All Subtype ELISA Kit (PBL Assay Science) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison